Purification and characterization of the membrane-bound ferrochelatase from Spirillum itersonii.

Base composition of deoxyribonucleic acid from Spirillum volutans, S. serpens and S. itersonii.

Cytochrome synthesis and its regulation in Spirillum itersonii.

Spirillum itersonii contains b- and c-type cytochromes as well as a carbon monoxide-binding pigment of the cytochrome o type. Synthesis of cytochromes b and c is increased by about two- and fourfold, respectively, when cells are transferred from high to low aeration. The increased concentration of cytochrome is not accompanied by an increase in the respiration rate of the cells. Both cytochrome...

Printed in Great Britain I so la t ion and Character iza t ion

The bacterium Spirillum itersonii has been used in studies of the regulation of cytochrome synthesis (Clark-Walker, Rittenberg & Lascelles, I967), but we felt that these studies would be aided if a transducing bacteriophage was available for genetic analysis. Attempts to isolate such a phage by induction with mitomycin C and ultraviolet light were unsuccessful, but examination of induced cultur...

ISOLATION AND PURIFICATION OF MAJOR OUTER MEMBRANE PROTEINS FROM BRUCELLA ABORTUS S-99

Isolation and purification of major outer membrane proteins (OMP) from the cell wall envelope of Brucella abortus S-99 were achieved by sonication, solubilization and membrane fractionation in the presence of non-ionic detergent (Tx-100) and lysozyme treatments, followed by ultracentrifugation. The crude OMP was treated with trypsin to free the preparation from any other protein contaminan...

Purification and properties of ferrochelatase from the yeast Saccharomyces cerevisiae. Evidence for a precursor form of the protein.

Ferrochelatase was purified to homogeneity from yeast mitochondrial membranes and found to be a 40-kDa polypeptide with a pI at 6.3. Fatty acids were absolutely necessary to measure the activity in vitro. The Michaelis constants for protoporphyrin IX (9 x 10(-8) M), ferrous iron (1.6 x 10(-7) M), and zinc (9 x 10(-6) M) were determined on purified enzyme preparations in the presence of dithioth...